Abstract

We are using SecM-derived translational arrest peptides to measure forces acting on the nascent chain during co-translational protein translocation and folding in vivo. The technique makes possible detailed studies of translocation and folding of both membrane proteins, secretory proteins, and cytoplasmic proteins.

References

Ismail, N., Hedman, R., Schiller, N., and von Heijne, G.A bi-phasic pulling force acts on transmembrane helices during translocon-mediated membrane integration. Nature Struct. Molec. Biol 19, 1018-1023.

Ismail, N., Hedman, R., Lindén, M., and von Heijne, G. (2015) Charge-driven dynamics of nascent chain movement through the SecYEG translocon. Nature Struct Molec Biol 22, 145-149.