PROGRAM

BN Seminar - Giovanni Maglia (RUG): 'Single-molecule enzymology and sequencing with nanopores'

Date:

Time:

16:00 hrs

 

Biological nanopores are a class of membrane proteins that form nanometer-size apertures on lipid membranes. Under an applied potential the ionic current through nanopores is used to identify molecules, or to follow reactions at the single-molecule level. Nanopores current are advantageous because they are easily integrated into low-cost and portable analytical devices.

 

Here we show that the transport of proteins and peptides across nanopores can be finely controlled. Folded proteins can trapped inside nanopores by exploiting electrophoretic forces, while small peptides can be stretched and unfolded by engineering strong electroosmotic flows through the nanopore. Using these approaches, nanopores may be used as nanoscale reactor for single-molecule enzymology studies, or sensors for sequencing individual proteins as they translocate across the nanopore.

 

 

 

Events

26 - 30 June 2017

BioBusiness Summer Schoollink

20 July 2017

Casimir Cafélink

21 - 25 August 2017

Topological Matter School 2017link

Workshops

29 March 2011 - 1 January 2018

Casimir Course: Hot Topicslink