BN seminar Joachim Spatz (Max Planck Inst. for Medical Research): "Sequential Bottom-up Assembly of Synthetic Cells"



16:00 hrs


A1.100, building 58, Delft


Sequential Bottom-up Assembly of Synthetic Cells

Joachim P. Spatz

Max Planck Institute for Medical Research, Heidelberg, Germany



The evolution of cellular compartments for spatially and temporally controlled assembly of biological processes became an essential step in developing life. Synthetic approaches towards cellular-like compartments are still lacking well-controlled functionalities as would be needed for more complex synthetic cells. In part, this is due to the mechanical and chemical instabilities of the lipid-based protocells and a lack of technical means for their well-controlled manipulation. We developed droplet supported lipid bilayer vesicles by microfluidics to generate mechanically and chemically stable and, therefore, manipulable cell-like compartments with a well-defined chemical and biophysical microenvironment. The enhanced stability enabled the sequential loading of such compartments with biomolecules by pico-injection microfluidics without compromising their functionality as synthetic cells. We demonstrate a successful sequential bottom-up assembly of a compartment with lipids, transmembrane proteins (integrin, FoF1-ATP synthase) and cytoskeleton proteins which would not assemble in a fully functional way by mixing and including them in one pot at once.