PROGRAM

BN seminar Joachim Spatz (Max Planck Inst. for Medical Research): "Sequential Bottom-up Assembly of Synthetic Cells"

Date:

Time:

16:00 hrs

Location:

A1.100, building 58, Delft

 

Sequential Bottom-up Assembly of Synthetic Cells

Joachim P. Spatz

Max Planck Institute for Medical Research, Heidelberg, Germany

http://www.mpimf-heidelberg.mpg.de/en

 

ABSTRACT

The evolution of cellular compartments for spatially and temporally controlled assembly of biological processes became an essential step in developing life. Synthetic approaches towards cellular-like compartments are still lacking well-controlled functionalities as would be needed for more complex synthetic cells. In part, this is due to the mechanical and chemical instabilities of the lipid-based protocells and a lack of technical means for their well-controlled manipulation. We developed droplet supported lipid bilayer vesicles by microfluidics to generate mechanically and chemically stable and, therefore, manipulable cell-like compartments with a well-defined chemical and biophysical microenvironment. The enhanced stability enabled the sequential loading of such compartments with biomolecules by pico-injection microfluidics without compromising their functionality as synthetic cells. We demonstrate a successful sequential bottom-up assembly of a compartment with lipids, transmembrane proteins (integrin, FoF1-ATP synthase) and cytoskeleton proteins which would not assemble in a fully functional way by mixing and including them in one pot at once.

Events

11 - 15 November 2019

Life Sciences with Industry 2019link