This course consists of 6 lectures covering various topics and several practicals that you can try yourself (though they won’t be adopted to your own microscope per se).
All Powerpoints and practical information can be found here
https://surfdrive.surf.nl/files/index.php/s/SXGXLeEtQKOjYRD
The lectures are also presented in an intereactive way online.
1. Physics of light
https://video.leidenuniv.nl/media/t/1_dcxitmkj
https://video.leidenuniv.nl/media/t/1_e38wkqm7
3. Contrast techniques
https://video.leidenuniv.nl/media/t/1_49ht4u6e
4. Histology and sample preparation
https://video.leidenuniv.nl/media/t/1_691gnymw
5. Fluorescence microscopy
https://video.leidenuniv.nl/media/t/1_9lonufca
6. Image analysis
https://video.leidenuniv.nl/media/t/1_ye02kigs
Certificate of participation
Pubquiz
For obtaining a certificate of participation please complete the following quiz (will be available during the course)
https://forms.gle/i5yTaYZD27Wijm589
Furthermore provide proof that you did the image analysis part by following the steps below
FIJI image analysis practical
- 1. Install FIJI
- 2. Go to: >Help>Update
- 3. Apply all needed changes
- 4. Restart FIJI
- 5. Repeat step 2 to 4 till you get the message “Your ImageJ is up to date!”
- NOTE: Sometimes you get an error message, just repeat the steps, it should work the 2nd time round
- 6. Click OK
- 7. Click Manage Update Sites
- 8. Select 3D ImageJ Suite, ImageScience, and Leiden University
- 9. Click Apply Changes
- 10. Restart FIJI
- 11. Go to: Plugins>LeidenUniv>Teaching>Interactive Tutorial
- 12. Finish all five assignments to get the Image analysis certificate
- 13. Email the proof to jwillemse@biology.leidenuniv.nl
